History

The OGI Genomics Core facility was established in April of 2013 by the Massachusetts Eye and Ear Department of Ophthalmology and with support of an NEI P30 grant (EY014104), with the goal of offering advanced genomics analysis methods and techniques to MEE investigators and affiliates.

Our Mission

Our team is committed to delivering high-quality, genomic services that address the evolving needs of the scientific community. We actively engage with researchers to understand their unique requirements and provide comprehensive tailored solutions from project planning to final analyses. Our goal is to ensure seamless integration of genomic technologies into research projects by offering a wide range of services from sample preparation through sequencing and analysis in collaboration with MEE Bioinformatics Core all in a CLIA-certified environment. We empower scientists to conduct impactful research across diverse disciplines.

Sanger sequencing is performed on our SeqStudioFlexgenetic analyzer delivering the highest quality data. High-quality pure basecallstypically have QV ≥20.OGI mean quality scores are QV 50.1 which correlates to a probability of error of 0.0010%. Average read lengths for plasmid sequencing is >1000bp with standard sequencing. We offer single-tube and high-throughput (96-well plate) formats with difficult template sequencing available in both formats.

Our NGS services include library preparation and short read sequencing. Library types are amplicon, whole plasmid, GEDi panel for ocular disease genes (RetNeT, glaucoma, optic neuropathy, mitochondrial DNA), whole genome sequencing, and RNA sequencing. We can also load your prepared libraries to your specifications on our Illumina Miseq or Novaseq6000 for sequencing only options.

Sample processing services include DNA/RNA extraction, plasma and serum isolation and buffy coat collection. All samples are accessioned and provided a unique barcode to ensure proper monitoring throughout the workflow by our LIMS. We provide secure storage and retrieval services for samples. Freezers are monitored 24/7, and staff are on-call to address any issues.
Instruments are available for use by investigators after training. These same services are available to be performed by our staff. Scheduling is through the MGB core website.

Acknowledgement

Please include the following statement in all publications and presentations from work provided by the OGI Genomics Core to assist us with NEI reporting and ensure our continued existence while acknowledging the valuable support provided by our core:

Sequencing and analysis were provided by the Ocular Genomics Institute (OGI) Genomics Core and was funded by the NIH National Eye Institute Core Grant P30EY014104.NIH National Eye Institute Core Grant P30EY014104.